Expression from the gonococcal cryptic plasmid is induced anaerobically. Schematic representations of (A) transcript-1 and (B) transcript-2 of the cryptic plasmid are displayed. Genes colored in grey are encoded on the positive strand, while genes colored in black are encoded on the negative strand. The predicted transcriptional start site, in parenthesis under the +1 for each transcript, is reported as the location on the plasmid according to the annotation of Korch et al., (1985) . Above each gene schematic, raw RNA-seq data from .wig files are plotted. The base count is representative of the number of times each base was mapped by a 50 bp RNA sequence read from Replicate 1 (normalized to take into account slight differences in total mapped reads between the two samples). Blue bars represent aerobic base reads and red bars represent anaerobic base reads. A prediction of -10 and -35 elements is given to the left of each schematic. RT-PCR confirms that transcript-1 is induced anaerobically.
Isabella and Clark BMC Genomics 2011 12:51 doi:10.1186/1471-2164-12-51