Open Access Research article

False negative rates in Drosophila cell-based RNAi screens: a case study

Matthew Booker13, Anastasia A Samsonova1, Young Kwon1, Ian Flockhart1, Stephanie E Mohr1 and Norbert Perrimon12*

Author Affiliations

1 Department of Genetics, Harvard Medical School, (77 Avenue Louis Pasteur), Boston, Massachusetts, (02115), USA

2 Howard Hughes Medical Institute, (77 Avenue Louis Pasteur), Boston, Massachusetts, (02115), USA

3 Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, (185 Meeting Street), Providence, Rhode Island, (02192), USA

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BMC Genomics 2011, 12:50  doi:10.1186/1471-2164-12-50

Published: 20 January 2011

Additional files

Additional data file 1:

Supplemental Table S1: dsRNA membership of the proteasome complex cluster and the cytosolic ribosome. Each gene is categorized as Proteasome, Ribosome, or Mitochondrial Ribosome. In bold are the 45 and 111 dsRNAs that belong to the proteasome and cytosolic ribosome clusters highlighted in Figure 2B and Figure 2A, respectively.

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Additional data file 2:

Supplemental Table S2: dsRNAs targeting the cytosolic ribosome and proteasome complex components that failed to appear in their respective clusters.

Format: XLS Size: 30KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data