Open Access Highly Accessed Research article

Nutrigenomics in honey bees: digital gene expression analysis of pollen's nutritive effects on healthy and varroa-parasitized bees

Cédric Alaux1*, Christelle Dantec2, Hughes Parrinello2 and Yves Le Conte1

Author Affiliations

1 INRA, UMR 406 Abeilles et Environnement, Domaine Saint-Paul, 84914 Avignon, France

2 Institut de Génomique Fonctionnelle, UMR5203 CNRS, U661 INSERM, Universités Montpellier 1 & 2, 34094 Montpellier Cedex 05, France

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BMC Genomics 2011, 12:496  doi:10.1186/1471-2164-12-496

Published: 10 October 2011

Additional files

Additional file 1:

Frequency of distinct aligned tags in the four DGE libraries.

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Additional file 2:

Lists of tags differentially expressed after pollen feeding or varroa parasitism. The number of copies found in each library, the sequence odds ratio and the Q-value are given for each tag. Corresponding honey bee gene and Drosophila ortholog are shown. Each tag ID is composed of the corresponding honey bee gene name and tag position in the gene (indicated by the last numbers). NaN stands for "not a number" and occurs when the denominator of the odds ratio is 0 (i.e., there are no sequences of a gene in the second tag library).

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Additional file 3:

Molecular function and biological process that were enriched in overlapping gene sets. A) Molecular function and biological process from genes that were upregulated in V-P+ but downregulated in V+P+. B) Molecular function and biological process from genes that were upregulated in V+P- but downregulated in V+P+. The number of genes differentially expressed in each pathway is shown.

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Additional file 4:

Molecular pathways affected by pollen feeding or varroa parasitism. Pathways that were significantly enriched (P < 0.05) in the different gene sets are shown. The analysis was done with DAVID 6.7 bioinformatic resources.

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Additional file 5:

Analysis with RT-PCR of selected immune and lifespan genes affected by pollen feeding or varroa parasitism. Expression values of selected lifespan (Sod, Sod2, Trxr-1) and immune genes (PGRP-LC, defensin1, Imd) differentially transcribed between the different treatments. RT-PCR data normalized to β-actin are shown. Means ± SE are shown for 8 pools of 3 bees per treatment. Different letters indicate significant differences detected by Mann-Whitney U tests.

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Additional file 6:

Sequences of qPCR primers.

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