Table 3

Regulation of transcripts of special interest in the short-term hypercapnia study on Carcinus maenas

CO2 experiments 3 + 7 days

CO2 experiments 11 weeks

Salinity experiments 7 days

[MA; med. log2-ratio ± MD]

[qPCR; med. log2-ratio ± MD]

[MA; mean log2-ratio ± SD]

rank

ACC. no.

Name

gill 7 - day 3

gill 9 -day 3

gill 7 - day 7

gill 9 -day 7

gill 7 - 10 weeks

gill 8 - day 7


1

DV944570.1

senescence-associated protein

-0.12 ± 0.50

-0.03 ± 0.03

    -1.16* ± 0.64

-0.07 ± 0.25

0.69 ± 0.57

-0.06 ± 0.03


2

DW250369.1

pg1 protein

1.01 ± 0.61

0.60 ± 1.14

-0.34 ± 0.49

-0.29 ± 0.56

-

1.644 ± 0.414


3

DN161290.1

unknown (putative Syntaxin binding protein 2)

0.32 ± 0.32

0.42 ± 0.10

    0.88* ± 0.10

0.67 ± 0.31

0.49 ± 0.66

2.86 ± 0.02


4

    DN796131.1

23S ribosomal RNA gene (a)

-1.20 ± 0.92

-0.25 ± 0.18

-1.14 ± 1.16

    -0.87* ± 0.46

-

0.36 ± 0.34


5

DW584691.1

calcium-activated chloride channel

0.25 ± 0.40

    0.81* ± 0.34

-0.09 ± 0.30

0.16 ± 0.31

1.12 ± 0.88

-


6

DN635040.1

23S ribosomal RNA gene (b)

-0.16 ± 1.42

0.09 ± 0.64

    -0.80* ± 0.22

-0.27 ± 0.49

-

1.29 ± 0.16


7

DV944193.1

rRNA intron-encoded homing endonuclease

0.54 ± 1.00

0.80 ± 0.81

0.06 ± 0.22

0.59 ± 0.41

-

1.073 ± 0.38


8

    DW249535.1

unknown

-0.12 ± 1.43

0.04 ± 0.55

    -0.71* ± 0.62

-0.41 ± 0.65

-

0.99 ± 0.29


9

    DY656451.1

unknown

-0.15 ± 0.23

0.05 ± 0.26

    0.57* ± 0.09

0.04 ± 0.27

-

-0.15 ± 0.30


10

    DW251049.1

unknown

-0.35 ± 0.24

    -0.53* ± 0.28

-0.05 ± 0.22

-0.10 ± 0.19

-

-0.86 ± 0.23


11

DN551127.1

unknown

0.54 ± 0.25

0.26 ± 0.14

    0.48* ± 0.31

0.37 ± 0.48

-

2.34 ± 0.07


12

    DW250828.1

thymosin-repeated protein 1

-0.39 ± 0.21

    -0.46* ± 0.14

0.13 ± 0.21

0.19 ± 0.21

-

-0.05 ± 0.06


13

    DN202592.1

multispanning membrane protein (transmembrane 9 superfamily protein member 4)

0.35 ± 0.23

0.21 ± 0.14

    0.46* ± 0.11

0.36 ± 0.34

-

2.15 ± 0.08


14

DW250260.1

hyperpolarization activated cyclic nucleotide-gated potassium channel-2

-0.26 ± 0.30

-0.14 ± 0.08

    -0.46* ± 0.24

    -0.14* ± 0.08

-0.13 ± 0.26

-0.11 ± 0.09


18

    DV944030.1

tetraspanin 3 (Transmembrane super family 4 member 8)

-0.22 ± 0.11

    -0.41* ± 0.05

0.01 ± 0.27

0.09 ± 0.03

-

-0.13 ± 0.06


25

    DY307809.1

potential integrin alpha-7

-0.12 ± 0.36

0.26 ± 0.21

    -0.35* ± 0.28

-0.03 ± 0.26

-

-


29

DV642643.1

kazal-type proteinase inhibitor

    0.25* ± 0.17

    0.21* ± 0.12

    0.33* ± 0.20

    0.24* ± 0.15

0.12 ± 0.84

1.04 ± 0.11


31

DN635395.1

unknown

    -0.17* ± 0.04

    -0.14* ± 0.04

    -0.32* ± 0.06

    -0.13* ± 0.06

0.25 ± 0.68

-0.11 ± 0.11


63

DV943872.1

predicted ATPase

-0.34 ± 0.33

-0.24 ± 0.10

-0.58 ± 0.39

    -0.23* ± 0.09

-

-0.65 ± 0.23


88

CX994129.1

anion/bicarbonate transporter family member (SLC family 4, member 1)

-0.31 ± 0.11

    -0.20* ± 0.09

-0.22 ± 0.07

-0.22 ± 0.11

0.18 ± 0.54

0.41 ± 0.11


-

DN739347.1

glycosyl-phosphatidylinositol-linked carbonic anhydrase VII

-0.09 ± 0.05

-0.06 ± 0.06

    -0.13* ± 0.07

    -0.08* ± 0.03

0.40 ± 0.35

-0.92 ± 0.07


-

    DN551450.1

glutathione peroxidase

0.02 ± 0.06

0 ± 0.05

    0.03* ± 0.02

-0.03 ± 0.05

-0.90 ± 0.24

-0.81 ± 0.14


-

DY656042.1

vacuolar-type H+-ATPase subunit A

-0.05 ± 0.37

0.11 ± 0.09

-0.11 ± 0.09

0.07 ± 0.06

0.45 ± 0.65

-0.17 ± 0.27


-

DY657461.1

v-type proton ATPase subunit E

0.02 ± 0.03

-0.02 ± 0.04

0 ± 0.03

-0.02 ± 0.03

0.30 ± 0.39

-0.12 ± 0.17


-

DW251095.1

cytoplasmic carbonic anhydrase

0.07 ± 0.01

-0.03 ± 0.06

0 ± 0.07

0.01 ± 0.01

-0.12 ± 1.20

1.78 ± 0.16


-

DN202373.1

anion/bicarbonate transporter family member (SLC family 4, member 11)

-0.02 ± 0.03

0.02 ± 0.06

-0.02 ± 0.10

0.01 ± 0.02

0.02 ± 0.64

-1.29 ± 0.47


-

DN796352.1

K+/Cl- symporter (solute carrier family 12, member 6)

-0.04 ± 0.04

0.22 ± 0.17

0.01 ± 0.04

-0.07 ± 0.07

0.91 ± 0.30

0.25 ± 0.23


-

DV111894.1

Na+/K+/2Cl- cotransporter

0.05 ± 0.05

0.01 ± 0.05

0 ± 0.06

0 ± 0.10

0.62 ± 0.57

-0.20 ± 0.09


-

DW250552.1

Na+/K+ ATPase alpha subunit

0.04 ± 0.05

0.02 ± 0.05

0.05 ± 0.10

0.03 ± 0.16

0 ± 1.11

1.38 ± 0.11


-

DY308103.1

Na+/H+ exchanger (NHE 3)

-

-

-

-

0.27 ± 0.03

-0.13 ± 0.14


Included are the 14 most strongly regulated transcripts (as identified by sign test and F-test), as well as several transcripts which have a potential function during structural modifications or in ion- and acid/base regulation. Transcripts are ordered according to the rank in absolute regulation. Accession numbers (ACC. no.) refer to the ESTs generated for the C. maenas microarray by Towle et al. [37] in the database GenBank (NCBI). Rank 1 = highest regulation; bold and underlined = significant regulation relative to the control identified by sign test; bold = identified to be differentially expressed by both tests; italic = identified to be differentially expressed only by F-test; "-" = a rank was not assigned in case the regulation was less than log2-ratio = |0.20|.

Fehsenfeld et al. BMC Genomics 2011 12:488   doi:10.1186/1471-2164-12-488

Open Data