Table 1

Sequence Mapping Statistics

Sample

Total reads

Primer

Mapped reads per primer

% of reads mapped

Total sequence per primer (Gb)

Total sequence (Gb)

Uniquely mapped reads

% of reads uniquely mapped

Uniquely mapped sequence (Gb)

Total uniquely mapped sequence (Gb)

Mean Coverage

% of targets with ≥ 8× coverage


ND02537_LCL

92248252

F3

F5

74121470

61566483

80.4%

66.7%

3.70

2.15

5.86

59773418

49605343

80.6%

80.6%

2.98

1.73

4.72

124.34

91.02%

ND02537_PBMC

93437335

F3

F5

73787463

61874203

79.0%

66.2%

3.68

2.16

5.85

59060679

49242442

80.0%

79.6%

2.95

1.72

4.67

123.07

90.34%

ND02538_LCL

83047764

F3

F5

66056191

56555527

79.5%

68.1%

3.30

1.97

5.28

53355146

45327323

80.8%

80.1%

2.66

1.58

4.25

111.95

91.20%

ND02538_PBMC

94548773

F3

F5

74721895

65.5%

79.0%

0.655

3.73

2.16

5.90

59775898

48973298

80.0%

79.1%

2.98

1.71

4.70

123.76

90.11%

ND02539_LCL

99014161

F3

F5

76834989

65785009

77.6%

66.4%

3.84

2.30

6.14

61133857

50648022

79.6%

77.0%

3.05

1.77

4.82

127.09

89.91%

ND02539_PBMC

98836019

F3

F5

81875758

68523012

82.8%

69.3%

4.09

2.39

66054927

54336918

80.7%

79.3%

3.30

1.90

5.20

136.96

90.21%

ND02540_LCL

93619368

F3

F5

77310874

65926759

82.6%

70.4%

3.86

2.30

6.17

63328774

52941514

81.9%

80.3%

3.16

1.85

5.01

132.09

90.12%

ND02540_PBMC

97278109

F3

F5

77375008

62335812

79.5%

64.1%

3.86

2.18

6.05

61792168

49552780

79.9%

79.5%

3.08

1.73

4.82

126.95

91.22%


Average

94003723

F3

F5

75260456

63059668

80.1%

67.1%

3.76

2.20

5.97

60534359

50078455

80.4%

79.4%

3.02

1.75

4.77

125.78

90.52%


Total reads: total number of sequence reads generated for the sample; Primer F3: 50 bp sequence; Primer F5: 35 bp sequence; Total sequence per primer: number of Gb of sequence generated for each primer; Uniquely mapped reads: number of reads which mapped only once to the genome; Total uniquely mapped reads: total Gb of uniquely mapped sequence generated; Mean coverage: average number of times each uniquely mapped base-pairs was sequenced

Londin et al. BMC Genomics 2011 12:464   doi:10.1186/1471-2164-12-464

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