Summary of the bioinformatic workflow followed. The raw sequence data was aligned to the hg19 human genome build (UCSC). Following the alignment of the sequence data, high-stringency parameters were used to make SNP and indel calls. Following the identification of genetic variants, the interpretation of our results included comparing the two-paired samples sequenced to determine if de novo mutations arise following EBV-transformation of B-lymphocytes.
Londin et al. BMC Genomics 2011 12:464 doi:10.1186/1471-2164-12-464