Table 5

Primers used for qRT-PCR analyses to validate deep sequencing data

Gene

Forward primer

Reverse Primer


Storage protein 1

CAAGACACGCTACGACGC

GTCGGCATGACGAAGTAC

Insulin receptor

GTACCCCTCGATCTCGCG

CCCACGTCAAGGGAACCC

JH epoxide hydrolase

AGGATCTACGCGGAGGGC

TGGTACACCACTTCGTAC

Ecdysteroid regulated protein

AACCCGAAGAGCCGAAGC

CTCTGTAGTCGCTGCTAC

Cuticle protein

CAGGATGACGAGTCTGGC

GTCTGCCTCGTATTCTAC

Toll receptor

CCTCCGGCAACGCCCTAG

CGCACAGAAATTCAGAGG

Hemolin

AGCTCCAGAGACTACGCC

GTGTTGTAGGAACCATTG

Gloverin

AGCTAGCCCGGCATCCGC

GACGGTAGCCCGCCTTAC

pxSerpin 3

GAATAGCTTCTACTACGC

TGATAGCGAATTCGGTAC

Actin

ATGGAGAAGATCTGGCAC

GGAGCCTCCGTGAGCAGC


Etebari et al. BMC Genomics 2011 12:446   doi:10.1186/1471-2164-12-446

Open Data