Table 3

Assessment of sensitivity and reproducibility at different miRNA input levels, spanning five orders of magnitude, Pearson correlation of duplicate measurementsĀ§.

The 125 spike-in miRNAs for which both platforms have assays

miRCURY

TaqMan

miRNA copies pr. PCR amplification

r (n detected)

r (n detected)


5,000/20,000

0.995 (124)

0.981 (123)

500/2,000

0.901 (124)

0.853 (121)

0.990 (116)*

50/200

0.920 (119)

0.930 (101)

5/20

0.829 (93)

0.843 (59)

0.5/2

0.228 (35)

0.249 (34)


Ā§ Calculated for all assays detected in both duplicates.

* Calculated after removing five assays with outlier measurements in one of the duplicates.

Jensen et al. BMC Genomics 2011 12:435   doi:10.1186/1471-2164-12-435

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