Table 2

Assessment of platform reproducibility, Pearson correlation of duplicate measurementsĀ§.

Sample

All assays detected

The 125 spike-in miRNAs for which both platforms have assays

miRCURY

TaqMan

miRCURY

TaqMan

r (n detected)

r (n detected)

r (n detected)

r (n detected)


Synthetic #1

0.991 (253)

0.936 (233)

0.968 (125)

0.947 (109)

Synthetic #2

0.993 (244)

0.892 (259)

0.988 (125)

0.886 (116)

Spiked plasma RNA #1

0.994 (309)

0.983 (308)

0.995 (124)

0.982 (123)

Spiked plasma RNA #2

0.992 (300)

0.989 (308)

0.990 (124)

0.989 (119)

Plasma

0.954 (114)

0.959 (99)

na

na

No-RT control

0.773 (8)

na (0)

na

na


Ā§ Calculated for all assays with Ct's below the detection threshold (TaqMan Ct = 30, miRCURY Ct = 38) in both duplicates.

Jensen et al. BMC Genomics 2011 12:435   doi:10.1186/1471-2164-12-435

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