Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs
Department of Molecular Medicine (MOMA), Aarhus University Hospital-Skejby, DK-8200 Aarhus N, Denmark
BMC Genomics 2011, 12:435 doi:10.1186/1471-2164-12-435Published: 26 August 2011
Additional file 1:
Table S1: Synthetic miRNAs and associated assays on the GeneChip, miRCURY, and TaqMan platforms. A list of the 174 synthetic miRNAs in the samples and the associated assays of the three investigated platforms.
Format: XLSX Size: 22KB Download file
Additional file 2:
Table S2A: miRNA assays on the TaqMan platform with homology to the synthetic miRNAs from pool A or B. Table S2B: miRNA assays on the miRCURY platform with homology to the synthetic miRNAs from pool A or B. Lists of assays on the TaqMan and miRCURY platforms with homology (≤ 4 nucleotides difference) to at least one of the synthetic miRNAs in the samples.
Format: XLSX Size: 33KB Download file
Additional file 3:
Figure S1 Assessment of reproducibility by scatter plots of the Ct values from replica experiments. Figure S2 Evaluation of whether the poorly recovered miRNAs of the TaqMan and miRCURY platforms were the same. The file contains the Figures S1 and S2.
Format: PDF Size: 440KB Download file
This file can be viewed with: Adobe Acrobat Reader