Open Access Highly Accessed Research article

Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs

Steffen G Jensen, Philippe Lamy, Mads H Rasmussen, Marie S Ostenfeld, Lars Dyrskjøt, Torben F Ørntoft and Claus L Andersen*

Author Affiliations

Department of Molecular Medicine (MOMA), Aarhus University Hospital-Skejby, DK-8200 Aarhus N, Denmark

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BMC Genomics 2011, 12:435  doi:10.1186/1471-2164-12-435

Published: 26 August 2011

Additional files

Additional file 1:

Table S1: Synthetic miRNAs and associated assays on the GeneChip, miRCURY, and TaqMan platforms. A list of the 174 synthetic miRNAs in the samples and the associated assays of the three investigated platforms.

Format: XLSX Size: 22KB Download file

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Additional file 2:

Table S2A: miRNA assays on the TaqMan platform with homology to the synthetic miRNAs from pool A or B. Table S2B: miRNA assays on the miRCURY platform with homology to the synthetic miRNAs from pool A or B. Lists of assays on the TaqMan and miRCURY platforms with homology (≤ 4 nucleotides difference) to at least one of the synthetic miRNAs in the samples.

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Additional file 3:

Figure S1 Assessment of reproducibility by scatter plots of the Ct values from replica experiments. Figure S2 Evaluation of whether the poorly recovered miRNAs of the TaqMan and miRCURY platforms were the same. The file contains the Figures S1 and S2.

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