Validation of the selected genes by quantitative PCR. Five days after adding dsRNA, the mRNA levels of selected genes were measured by real time RT-PCR using rp49 for normalization. The y axis represents the relative mRNA level of each gene in the cells treated SPS1 dsRNA (SPS1i) to that treated GFP dsRNA (GFPi). The mRNA level of GFPi was set to 100%. The gene symbol is marked above each graph.
Lee et al. BMC Genomics 2011 12:426 doi:10.1186/1471-2164-12-426