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Open Access Highly Accessed Research article

Combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem mass spectrometry as an effective strategy for analyzing potential membrane protein complexes of Mycobacterium bovis bacillus Calmette-Guérin

Jianhua Zheng, Candong Wei, Lina Zhao, Liguo Liu, Wenchuan Leng, Weijun Li and Qi Jin*

Author Affiliations

State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China

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BMC Genomics 2011, 12:40  doi:10.1186/1471-2164-12-40

Published: 18 January 2011

Additional files

Additional file 1:

List of the identified proteins by MS analysis showing the sequence of all the identified peptides by independent MS/MS fragmentation. The charge state of each peptide is +1. Accession: the identifier number from the National Centre for Biotechnology Information database; Protein ID: protein name in M. bovis BCG database; Score: the Mascot score of the protein identification; MW [kDa]: molecular weight in kDa of the respective protein; pI: isoelectric point of the protein, calculated from its amino acid sequence; SC[%]: sequence coverage in percent, calculated from the identified peptides of the respective protein; RMS [ppm]: the RMS value of the deltas between the calculated and experimental masses of the peptides, which belong to a particular protein; Inten1.: sum of all intensity values related to 100 laser shots for all peptides, which belong to a particular protein; S/N1: sum of all S/N-values for all peptides, which belong to a particular protein; MH+ (calc) [Da]: calculated mass of the singly protonated peptide; Δm [Da]: difference between the measured and calculated masses of the respective peptide in Dalton; Inten2: the sum of the MS peak intensities per 100 laser shots; S/N2: S/N ratio of the respective peptide; Sequence: the sequence of the corresponding peptide.

Format: XLS Size: 184KB Download file

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