Combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem mass spectrometry as an effective strategy for analyzing potential membrane protein complexes of Mycobacterium bovis bacillus Calmette-Guérin
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China
BMC Genomics 2011, 12:40 doi:10.1186/1471-2164-12-40Published: 18 January 2011
Additional file 1:
List of the identified proteins by MS analysis showing the sequence of all the identified peptides by independent MS/MS fragmentation. The charge state of each peptide is +1. Accession: the identifier number from the National Centre for Biotechnology Information database; Protein ID: protein name in M. bovis BCG database; Score: the Mascot score of the protein identification; MW [kDa]: molecular weight in kDa of the respective protein; pI: isoelectric point of the protein, calculated from its amino acid sequence; SC[%]: sequence coverage in percent, calculated from the identified peptides of the respective protein; RMS [ppm]: the RMS value of the deltas between the calculated and experimental masses of the peptides, which belong to a particular protein; Inten1.: sum of all intensity values related to 100 laser shots for all peptides, which belong to a particular protein; S/N1: sum of all S/N-values for all peptides, which belong to a particular protein; MH+ (calc) [Da]: calculated mass of the singly protonated peptide; Δm [Da]: difference between the measured and calculated masses of the respective peptide in Dalton; Inten2: the sum of the MS peak intensities per 100 laser shots; S/N2: S/N ratio of the respective peptide; Sequence: the sequence of the corresponding peptide.
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