Table 5 |
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|
A validation of Toll-like receptors and IFN-stimulated genes by qRT-PCR |
|||
|
Gene |
GenBank ID |
Microarray FC |
qRT-PCR FC |
|
|
|||
|
TLR2 |
2.87 |
4.61 |
|
|
TLR3 |
7.33 |
3.42 |
|
|
TLR8 |
2.68 |
2.44 |
|
|
TLR9 |
2.20 |
1.64 |
|
|
IRF3 |
4.32 |
6.33 |
|
|
IRF7 |
3.25 |
11.1 |
|
|
Mx1 |
3.24 |
10.36 |
|
|
ISG15 |
3.45 |
5.21 |
|
|
ISG20 |
5.41 |
2.15 |
|
|
ISG54 |
2.73 |
6.27 |
|
|
ISG56 |
3.39 |
3.43 |
|
|
ISG60 |
2.47 |
5.70 |
|
|
GBP1 |
2.78 |
12.0 |
|
|
OASL |
5.80 |
2.05 |
|
|
OAS2 |
2.05 |
28.80 |
|
|
|
|||
|
qRT-PCR results of 4 Toll-like receptors and 11 IFN-stimulated genes in the SwIV -infected pigs compared with the controls. Gene expression is depicted as relative expression compared to GAPDH gene expression. |
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|
Li et al. BMC Genomics 2011 12:398 doi:10.1186/1471-2164-12-398 |
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