Table 2

HPLC-MS/MS analysis of seed coat extracts of black (iRT) and brown (irT) soybean

Compound

Identity

[M+] (m/z)

Major fragment(s)

Rt (min)

Genotypea


1

Unknown 1

413

183, 115

1.4

B

2

Unknown 2

381

184, 125

1.6

irT

3

Unknown 3

183

-

1.7

iRT

4

Unknown 4

162

-

1.9

B

5

Unknown 5

409

303

1.9

B

6

Unknown 6

437

273

2.3

B

7

Unknown 7

416

-

2.3

B

8

Unknown 8

580

182, 265, 437

2.5

B

9

Unknown 9

507

485, 323, 132

2.9

B

10

Orcinol O-hexoside

305

287

4.0

irT

11

Unknown 10

331

248

4.2

irT

12

Procyanidin trimer 1

867

579, 309, 299

4.7

iRT

13

Procyanidin B2*

579

291

5.5

B

14

Unknown 11

607

409, 455

6.0

B

15

Procyanidin trimer 2

867

577, 407

6.4

B

16

Delphinidin-3-O-glucoside*

465

303

6.6

iRT

17

Procyanidin trimer 3

867

579

6.6

iRT

18

Epicatechin*

291

-

6.9

B

19

Cyanidin-3-O-galactoside*

449

287

7.1

iRT

20

Daidzein O-hexoside malonylated

503

417

7.4

irT

21

Unknown 12

481

319

7.4

irT

22

Cyanidin-3-O-glucoside*

449

287

7.5

iRT

23

Petunidin-3-O-glucoside*

479

319, 303

8.0

iRT

24

Unknown 13

341

278, 111

8.1

irT

25

Pelargonidin-3-O-glucoside*

433

-

8.3

iRT

26

Peonidin-3-O-glucoside*

463

301, 286

8.7

iRT

27

Procyanidin dimer 2

579

409, 291

9.2

B

28

Unknown flavonol 1

331

-

9.0

irT

29

Procyanidin trimer 4

867

579, 699

9.2

iRT

30

A-type procyanidin dimer

593

-

9.7

iRT

31

Unknown anthocyanin 1

317

-

9.6

iRT

32

Unknown flavonol 2

615

-

9.6

irT

33

Cyanidin*

287

-

10.0

iRT

34

Unknown 14

487

-

10.8

B

35

Diosmetinidin*

285

-

10.3

iRT

36

Unknown benzoic acid 1

633

331, 315

11.0

irT

37

Quercetin-3-O-glucoside*

465

303

11.0

Bb


*Based on co-chromatographic and MS fragamentation comparison to an authentic standard.

aBlack soybean (iRT), brown soybean (irT), both (B).

bTrace amounts in iRT.

Kovinich et al. BMC Genomics 2011 12:381   doi:10.1186/1471-2164-12-381

Open Data