Additional file 2.

Supplementary Figure S1. Proanthocyanidin (PA) subunit compositions, degree of polymerizations, and amounts from the seed coats of black (iRT) and brown (irT) soybean Clark isolines. (A, B, C) iRT top panels, irT bottom panels. (A) Phloroglucinol cleavage products of soluble PA polymers. HPLC retention times: ascorbic acid (1) (Rt: 2.0 min); phloroglucinol (2) (Rt: 3.1 min); epicatechin-phloroglucinol adduct (Rt: 5.5 min); epicatechin (4) (Rt: 9.4 min). (B) Phloroglucinol cleavage products of solvent insoluble PA polymers. HPLC retention times: ascorbic acid (1) (Rt: 2.0 min); phloroglucinol (2) (Rt: 3.1 min); epicatechin-phloroglucinol (Rt: 6.2 min); epicatechin (4) (Rt: 11.0 min). (C) Free monomers. HPLC retention times: epicatechin (1) (Rt: 12.5 min). (D) Mean degree of polymerization (mDP) of soluble PAs. (E) Total insoluble PAs. (E) Total soluble PAs. (D, E, F) iRT black bars, irT white bars. (E, D) Amounts are represented as milligrams procyanidin B2 equivalents per gram lyophilized seed coat (LSC).

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Kovinich et al. BMC Genomics 2011 12:381   doi:10.1186/1471-2164-12-381