Purification of his-tagged OMT5 and determination of the reaction product for cyanidin 3-O-glucoside. (A) SDS-PAGE analysis of OMT5: total soluble protein from E. coli expressing OMT5 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified OMT5 (lane 3). (B) The OMT5 enzyme reaction as revealed by HPLC-DAD chromatograms at 520 nm (C). HPLC retention times: cyanidin 3-O-glucoside (Rt: 7.3 min); peonidin 3-O-glucoside (Rt: 8.6 min).
Kovinich et al. BMC Genomics 2011 12:381 doi:10.1186/1471-2164-12-381