Table 2

Comparison of primer sequences between OsBRI1 and its switchgrass homologsa

Primer

S1

S2

S3


Rice

TGGAGAC

    C
ATTGGCAAGATC

GC

    G
A
    TT
TTCA
    AA
TGCTCC
    A
G

TCCA

    A
GCA
    C
GCACA
    G
GC
    G

BAC 48E09 (D10) b

TGGAGAC

    T
ATTGGCAAGATC

GC

    A
A
    CC
TTCA
    AG
TGCTCC
    A
G

TCCA

    A
GCA
    A
GCACA
    A
GC
    A

BAC 65J23 (E2) b

TGGAGAC

    C
ATTGGCAAGATC

GC

    A
A
    CC
TTCA
    GG
TGCTCC
    A
G

TCCA

    G
GCA
    A
GCACA
    A
GC
    A


aPrimers were designed based on sequence of the hypothetically conserved region of the rice OsBRI1 gene

bThe corresponding sequences of the switchgrass homologs of the OsBRI1 gene in two homoeologous BACs are also listed. The different nucleotides are underlined.

Saski et al. BMC Genomics 2011 12:369   doi:10.1186/1471-2164-12-369

Open Data