Open Access Highly Accessed Research article

Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids

Yu-Yun Hsiao12, Yun-Wen Chen3, Shi-Ching Huang1, Zhao-Jun Pan1, Chih-Hsiung Fu4, Wen-Huei Chen2, Wen-Chieh Tsai23* and Hong-Hwa Chen123*

Author Affiliations

1 Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan

2 Orchid Research Center, National Cheng Kung University, Tainan 701, Taiwan

3 Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan 701, Taiwan

4 Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan

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BMC Genomics 2011, 12:360  doi:10.1186/1471-2164-12-360

Published: 12 July 2011

Additional files

Additional file 1:

Length distribution of assembled contigs and singletons. This table summarizes the number of contigs and singletons in different length distribution.

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Additional file 2:

Summary of component reads per assembly. This table summarizes the number of component reads assembled into contigs.

Format: DOC Size: 27KB Download file

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Additional file 3:

Gene Families identified by BLAST annotation of Phalaenopsis transcriptome. This table summarizes the BLAST results of all Unigenes against Arabidopsis proteome and then categorized by Arabidopsis gene families.

Format: DOC Size: 151KB Download file

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Additional file 4:

Expressed sequence tags with substantial similarity to terpenoid backbone biosynthetic genes. This table summarizes the number of unigenes and reads in each step of terpenoid biosynthetic pathway.

Format: DOC Size: 128KB Download file

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