Figure 4.

Silencing targets identified from the primary screen. CL3^EcoR cells were infected in triplicate with lentiviruses prepared from the indicated GIPZ shRNAmir constructs targetting TMEM9B (a), LTBP2/3 (b), ATXN10 (c) and LAYN (d). Cells resistant to 6 μg/ml puromycin were isolated, reseeded and assayed for bypass of senescence by culturing at 38°C for 3 weeks. Lentiviruses prepared from the GIPZ shRNAmir Lamin A/C (v2LHS_62719) construct were used as the negative control. For TMEM9B, the mix of constructs comprised v2LHS_247318, 58957, 58958 and 58959.