Sensitivity of the screen. CL3EcoR cells were infected with retroviruses prepared from pRSLamin A/C (a), pRSp21F (b), or a 1/200 mix of pRSp21F/pRSLamin A/C (c). After puromycin selection, cells were reseeded at 8.5 × 104 per 15 cm plate or 0.5 × 104 per well in 6-well plates and shifted to 38°C for 3 weeks.
Rovillain et al. BMC Genomics 2011 12:355 doi:10.1186/1471-2164-12-355