Open Access Highly Accessed Research article

An RNA interference screen for identifying downstream effectors of the p53 and pRB tumour suppressor pathways involved in senescence

Emilie Rovillain1, Louise Mansfield1, Christopher J Lord2, Alan Ashworth2 and Parmjit S Jat1*

Author Affiliations

1 Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK

2 Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research,237 Fulham Road, London SW3 6JB, UK

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BMC Genomics 2011, 12:355  doi:10.1186/1471-2164-12-355

Published: 8 July 2011

Additional files

Additional file 1:

Volume of virus supernatants used for the screen. The table shows the volume of virus supernatants used for each of the pools.

Format: XLS Size: 20KB Download file

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Additional file 2:

Primary screen. This table shows the pool number, the number of cells obtained after puromycin selection, the number of T-75 and T-25cm2 flasks reseeded and the number of growing colonies observed after 3 weeks at 38°C. Numbers indicated in red correspond to flasks that were reseeded for extracting genomic DNA.

Format: XLS Size: 40KB Download file

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Additional file 3:

Unidentified inserts. This table shows the hair pin sequences that were not 100% homologous to a gene by BLASTN analysis of the NCBI human genome data base (http://blast.ncbi.nlm.nih.gov/Blast.cgi webcite) or could not be linked back to a hair pin sequence within the Open Biosystems SM2 library; the number of times the insert was isolated is also indicated.

Format: XLS Size: 32KB Download file

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