"Excision assay" performed with genomic DNA isolated from 2-day old zebrafish embryos: GFP expression in the embryos injected with iTol2-BACs were observed between 28 and 48 hours post fertilization (hpf) (upper panels). APPb:EGFP BAC 13 (panel 5A), APPb:EGFP BAC 2 (panel 5B), fgf24:EGFP BAC 37 (panel 5C) and fgf24:EGFP BAC 35 (panel 5D) display green fluorescence. The white arrowheads indicate the expected tissue-specific expressions of GFP in these embryos. The iTol2-BAC injected embryos were analyzed using the "excision assay" (lower panels) [16,4].
Shakes et al. BMC Genomics 2011 12:351 doi:10.1186/1471-2164-12-351