Methodology article
Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons
1 Julius L. Chambers Biomedical/Biotechnology Research Institute & Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
2 Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
3 Department of Chemistry, University of North Carolina at Chapel-Hill, Chapel-Hill, NC 27599. USA
BMC Genomics 2011, 12:351 doi:10.1186/1471-2164-12-351
Published: 7 July 2011Additional files
Additional file 1:
Location of iTol2kan insertions (new BAC ends) in APPb:EGFP and fgf24:EGFP BACs. Newly created ends containing the iTol2kan cassette were sequenced with primers Tkan1 and Tkan2 for APPb:EGFP BAC deletions (Panel A), and with primer Seq1 for fgf24:EGFP BAC deletions (Panel B). The sequences were BLASTed to the zebrafish genome, and location of the new ends of BACs where the iTol2kan cassettes are placed indicated in panels A and B. Clone numbers are as in lanes shown by the blue arrowheads in Figure 4.
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