Open Access Highly Accessed Methodology article

Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons

Leighcraft A Shakes1, Gembu Abe2, Mugtaba A Eltayeb1, Hope M Wolf13, Koichi Kawakami2 and Pradeep K Chatterjee1*

Author Affiliations

1 Julius L. Chambers Biomedical/Biotechnology Research Institute & Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA

2 Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan

3 Department of Chemistry, University of North Carolina at Chapel-Hill, Chapel-Hill, NC 27599. USA

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BMC Genomics 2011, 12:351  doi:10.1186/1471-2164-12-351

Published: 7 July 2011

Additional files

Additional file 1:

Location of iTol2kan insertions (new BAC ends) in APPb:EGFP and fgf24:EGFP BACs. Newly created ends containing the iTol2kan cassette were sequenced with primers Tkan1 and Tkan2 for APPb:EGFP BAC deletions (Panel A), and with primer Seq1 for fgf24:EGFP BAC deletions (Panel B). The sequences were BLASTed to the zebrafish genome, and location of the new ends of BACs where the iTol2kan cassettes are placed indicated in panels A and B. Clone numbers are as in lanes shown by the blue arrowheads in Figure 4.

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