Table 3

O157:H7-Specific Gene Targets

    ProbeSet ID

    Accession ID

    Strain

    Genome Position

    Locus

    Gene

    Product

    GI


1766456_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2849958-2851076

Z3198-RC

-

EDL933

16445223

1759686_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2848990-2849955

Z3197

fcI

fucose synthetase

962092

1759686_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2778776-2779741

ECs2838

-

fucose synthetase

912293

1766456_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2779744-2780862

ECs2839

-

GDP-D-mannose dehydratase

912548

1766456_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2849958-2851076

Z3198

-

GDP-mannose dehydratase

962093

1764806_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2848478-2848987

Z3196

wbdQ

GDP-mannose mannosylhydrolase

962091

1762793_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2780369-2780601

ECs5479

-

hypothetical protein

2693774

1759443_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2776834-2778282

ECs2836

-

mannose-1-P guanosyltransferase

912820

1759443_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2847048-2848496

Z3195

manC

mannose-1-P guanosyltransferase

962090

1762953_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2783218-2784603

ECs2842

-

O antigen flippase

912601

1762953_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2853432-2854823

Z3201

wzx

O antigen flippase Wzx

962096

1766849_s_at

NC_002655.2

Escherichia coli O157:H7 EDL933

2855525-2856709

Z3203

wzy

O antigen polymerase

962098

1766849_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2785311-2786495

ECs2844

-

O antigen polymerase

912486

1764806_s_at

NC_002695.1

Escherichia coli O157:H7 Sakai

2778264-2778773

ECs2837

-

putative GDP-L-fucose pathway enzyme

912421


Using the MAS 5.0 gene detection method, we filtered those probe sets that were consistently called "present" in all O157:H7 strains yet were called "absent" in all non-O157:H7 strains. The 14 probe sets shown here correspond to O157:H7-specific genes.

Jackson et al. BMC Genomics 2011 12:349   doi:10.1186/1471-2164-12-349

Open Data