Open Access Research article

Transcriptomic response of the red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition

Jeanine S Morey1, Emily A Monroe13, Amanda L Kinney1, Marion Beal1, Jillian G Johnson1, Gary L Hitchcock2 and Frances M Van Dolah1*

Author Affiliations

1 Marine Biotoxins Program, NOAA National Ocean Service, Center for Coastal Environmental Health and Biomolecular Research, 219 Fort Johnson Rd., Charleston, SC 29412, USA

2 Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, 4600 Rickenbacker Cswy., Miami, FL 33149, USA

3 Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Dr. La Jolla, CA 92093, USA

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BMC Genomics 2011, 12:346  doi:10.1186/1471-2164-12-346

Published: 5 July 2011

Additional files

Additional file 1:

Primers used for real-time PCR. This pdf file contains the contig number, sequence description, forward and reverse primer sequences, and annealing temperatures of all genes validated by qPCR in this study.

Format: PDF Size: 7KB Download file

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Open Data

Additional file 2:

Annotated genes in the nitrogen addition trend set. This pdf file contains the contig number, sequence description, BLASTx e-value, cluster number, and fold change and p-values for all annotated genes in the nitrogen addition trend set.

Format: PDF Size: 32KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Genes from the N-addition trend set in the enriched GO terms involved in amino acid biosynthesis/metabolism, sulfate assimilation and reduction, or nitrogen compound biosynthetic processes. This pdf file contains the contig number, sequence description, BLASTx e-value, and fold change and p-values for the genes in the N-addition trend set resulting in enrichment of GO terms involved in amino acid biosynthesis/metabolism, sulfate assimilation and reduction, or nitrogen compound biosynthetic processes.

Format: PDF Size: 10KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Annotated genes in the phosphorus addition trend set. This pdf file contains the contig number, sequence description, BLASTx e-value, cluster number, and fold change and p-values for all annotated genes in the phosphorus addition trend set.

Format: PDF Size: 28KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data