Open Access Highly Accessed Research article

Gatekeeper of pluripotency: A common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells

Maurizio Zuccotti1*, Valeria Merico2, Michele Bellone3, Francesca Mulas4, Lucia Sacchi5, Paola Rebuzzini3, Alessandro Prigione6, Carlo A Redi2, Riccardo Bellazzi45, James Adjaye6 and Silvia Garagna347*

Author Affiliations

1 Sezione di Istologia ed Embriologia, Dipartimento di Medicina Sperimentale, Universita' degli Studi di Parma, Parma, Italy

2 Fondazione I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

3 Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy

4 Centro di Ingegneria Tissutale, Universita' degli Studi di Pavia, Pavia, Italy

5 Dipartimento di Informatica e Sistemistica, Universita' degli Studi di Pavia, Pavia, Italy

6 Molecular Embryology and Aging Group, Department of Vertebrate Genomics. Max-Planck Institute for Molecular Genetics, Berlin, Germany

7 Centro di Eccellenza in Biologia Applicata, Universita' degli Studi di Pavia, Pavia, Italy

For all author emails, please log on.

BMC Genomics 2011, 12:345  doi:10.1186/1471-2164-12-345

Published: 5 July 2011

Additional files

Additional file 1:

GO enrichment. List of regulated genes (MIINSN vs. MIIctrl).

Format: XLS Size: 319KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

GO enrichment. List of regulated genes (2-cellNSN vs. 2-cellctrl)

Format: XLS Size: 195KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

qRT-PCR expression profile of Oct4 and five Oct4-regulated genes. This analysis confirmed the down-regulated pattern of expression detected by microarray analysis when comparing 2-cellNSN vs. 2-cellctrl embryos.

Format: JPEG Size: 176KB Download file

Open Data

Additional file 4:

Gene annotation similarity network. Gene annotation similarity network made of 312 genes retrieved when exploring the public databases for GO and MeSH links between the group of 32 Oct4-regulated genes and all the annotated mouse gene sequences. Green lines, MeSH annotations; orange lines, MeSH and GO annotations; grey lines, GO annotations. Increasing line width indicates stronger annotation relationship. Red dot, Oct4 gene.

Format: JPEG Size: 1.5MB Download file

Open Data

Additional file 5:

Oct4 transcriptional network in MII oocytes. Genes expressed exclusivelly in MII oocytes. Green box, down-regulated; red box, up-regulated.

Format: XLS Size: 24KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 6:

Oct4 transcriptional network in 2-cell embryos. Genes expressed exclusivelly in 2-cell embryos. Green box, down-regulated; red box, up-regulated.

Format: XLS Size: 19KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 7:

Main characteristics of the Oct4-OETN genes. Main characteristics of the genes found in each of the 18 gene clusters.

Format: DOC Size: 49KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 8:

List of cancer-related genes present in the expanded Oct4-TN. The majority of the genes belonging to the expanded Oct4-TN were recognised as cancer-related genes.

Format: XLS Size: 15KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additionalfile 9:

List of cancer-related genes present in the Oct4-OETN. The majority of the genes belonging to the Oct4-OETN were recognised as cancer-related genes.

Format: XLS Size: 18KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 10:

Microarray analysis. Microarray analysis of 2-cell embryos.

Format: XLS Size: 8MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 11:

RT-PCR primers. List of primers used for the real time RT-PCR analysis.

Format: DOC Size: 31KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data