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Open Access Highly Accessed Research article

Identification of lignin genes and regulatory sequences involved in secondary cell wall formation in Acacia auriculiformis and Acacia mangium via de novo transcriptome sequencing

Melissa ML Wong1, Charles H Cannon23 and Ratnam Wickneswari1*

Author Affiliations

1 School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi 43600, Selangor, Malaysia

2 Ecological Evolution Group, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Science, Menglun, Mengla 666303, Yunnan, P. R. China

3 Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409 USA

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BMC Genomics 2011, 12:342  doi:10.1186/1471-2164-12-342

Published: 5 July 2011

Additional files

Additional file 1:

Multiple protein sequence alignments of monolignol biosynthetic genes in Arabidopsis thaliana, A. auriculiformis and A. mangium. The file provides the multiple protein sequence alignments of all ten monolignol biosynthetic genes detected in A. auriculiformis and A. mangium with corresponding A. thaliana genes. Conserved motifs are highlighted in colour.

Format: DOC Size: 91KB Download file

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Open Data

Additional file 2:

Genbank accession numbers of monolignol biosynthetic genes in A. auriculiformis and A. mangium. The table provides the lengths and accession numbers for the assembled sequences of monolignol biosynthetic genes from A. auriculiformis and A. mangium that were deposited in NCBI Transcriptome Shortgun Assembly (TSA).

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Open Data

Additional file 3:

Multiple protein sequence alignments of R2R3-MYBs in A. auriculiformis and A. mangium and other species used in phylogenetic tree construction. R2 and R3 repeats are shown.

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Open Data

Additional file 4:

Primer pairs for miRNA stem-loop sequences in A. auriculiformis. The table shows the list of primer sequences with product size and annealing temperature used in the amplification of miR160, miR172 and miR396 stem-loop sequencing in A. auriculiformis.

Format: DOC Size: 30KB Download file

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Open Data