Additional file 9.
Details of copy number calculation with Real time PCR. Standard curve based on 10 fold dilution series of pDp1, pDp2, pDp3, pDp4 and genomic DNA from buffalo, cattle, goat and sheep showing the amplification plot (a-d) panel (A), corresponding slopes of -3.3 to -3.5, panel (B) and a single dissociation peak, panel (C), substantiating maximum efficiency of the PCR reaction and high specificity of the primers with target DNA. Arrow indicates genomic DNA from buffalo, cattle, goat and sheep.
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Pathak and Ali BMC Genomics 2011 12:338 doi:10.1186/1471-2164-12-338