Validation by category, direct comparison examples. Only the largest data sets are shown. (A) Validation in a single animal with multiple chemistries. All data for the reference animal 17573 are displayed by chemistry. SNPs falling into the overlap region are considered to be validated. (B) Validation in multiple animals with a single chemistry and with multiple chemistries. SOLiD data from re-sequencing efforts were compared to the unrelated animals r1766 and r02120. A large number (603,463) of additional SNPs not identified in the reference animal were identified and validated by comparing r02120and r1766. We observed roughly one-third the number of validated SNPs from r02120 that were obtained using the sequence data from r1766, due to reduced uniquely mapping sequence coverage in r02120 (Table 1).
Fawcett et al. BMC Genomics 2011 12:311 doi:10.1186/1471-2164-12-311