Open Access Research article

Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon

Kristian R von Schalburg1, Motoshige Yasuike1, Ryosuke Yazawa2, Johan G de Boer1, Linda Reid1, Stacy So1, Adrienne Robb1, Eric B Rondeau1, Ruth B Phillips3, William S Davidson4 and Ben F Koop1*

Author Affiliations

1 Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, V8W 3N5, Canada

2 Tokyo University of Marine Science and Technology, Tateyama, Chiba, 294-0308, Japan

3 Biological Sciences, Washington State University, Vancouver, Washington, 98686-9600, USA

4 Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada

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BMC Genomics 2011, 12:31  doi:10.1186/1471-2164-12-31

Published: 13 January 2011

Additional files

Additional file 1:

Graphical presentation of number of mortalities found throughout early development. Day represents post-fertilization age.

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Additional file 2:

cDNA expression profiles for ten genes of interest in twelve different adult tissues. cDNA expression profiles for ten genes of interest (GOI) in twelve different tissues: 1: kidney, 2: muscle, 3: skin, 4: gut, 5: gill, 6: spleen, 7: brain, 8: heart, 9: testis, 10: liver, 11: eye and 12: pyloric caecum. Semi-quantitative levels of expression were calculated for each GOI as signal intensity relative to ubiquitin levels for three individual fish. Mean values (bars) of three individuals plus standard error are shown.

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Additional file 3:

Alignment of partial mis transcripts. Sequence alignment of mis cDNAs and amino acid residues based on sequence upstream from the C-terminal hormone portion of salmon MIS. A. The stop codon for mis (629 bp) is highlighted in red. The open reading frame of mis (396 bp) cDNA is in-frame for encoding the bioactive hormone portion of MIS and does not contain a stop codon. Two potential protease recognition motifs (RGQR AND RATR) for translated MIS (396 bp) are boxed. B. The stop codons for mis (629 bp) and mis (436 bp) are highlighted in red. Putative cleavage RLRR recognition sites are boxed.

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Additional file 4:

Proximal promoter sequences of the Atlantic salmon dax1 gene. The potential binding elements of various transcription factors, TATA boxes, exon 1 and initiator methionine codon are labeled.

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Additional file 5:

Proximal promoter sequences of the Atlantic salmon dax2 gene. The potential binding elements of various transcription factors, TATA boxes, exon 1 and initiator methionine codon are labeled.

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Additional file 6:

Proximal promoter sequences of the Atlantic salmon mis gene. The potential binding elements of various transcription factors, TATA boxes, exon 1 and initiator methionine codon are labeled.

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Additional file 7:

Proximal promoter sequences of the Atlantic salmon foxl2b gene. The potential binding elements of various transcription factors, TATA boxes, exon 1 and initiator methionine codon are labeled.

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Additional file 8:

Proximal promoter sequences of the Atlantic salmon pseudogenic foxl2a gene. Potential TATA boxes and the 5'-end portion of a region of sequence similarity shared with rainbow trout foxl2 genes are labeled.

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Additional file 9:

Fluorescent in situ hybridization of dax1-containing BAC.

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Additional file 10:

Fluorescent in situ hybridization of dax2-containing BAC.

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Additional file 11:

Fluorescent in situ hybridization of mis-containing BAC.

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Additional file 12:

Primer oligonucleotide sequences used in RT-PCRs.

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