Genes discovered in the first replicate had higher NSAF (arcsin square root transformed) than genes discovered in later replicates. This pattern was seen in all four biological samples; we present one of the copulatory plug samples here. This result suggests that we have identified all reasonably abundant (and detectable) proteins under the experimental conditions employed.
Dean et al. BMC Genomics 2011 12:306 doi:10.1186/1471-2164-12-306