Measurement of global percentage of methylated DNA in zebrafish and calf using HPLC. A. Determination of the retention time of five standard mononucleotides using HPLC. An arrow indicates absence of uracil peak showing that the sample is not contaminated with RNA. B. Percentage of DNA methylated in healthy zebrafish (D.rerio) and calf (B.taurus) thymus. Global percentage of methylated DNA in zebrafish is 2.14 fold higher than calf. Data are the mean ± SEM of three independent experiments. ** Significantly different from calf (p < 0.01).
Mirbahai et al. BMC Genomics 2011 12:3 doi:10.1186/1471-2164-12-3