Table 2

Summary statistics for reference transcriptome through progressive stages of assembly.

Assembly stage

N50

Mean

contig size

Total

length

Number

of contigs


Oases assembly

611

374.1

21,311,238

56,971

Reciprocal BLAST

807

703.5

15,919,812

22,630

Contig elongation

812

709.9

16,065,194

22,630

Reduced fragmentation

819

717.8

15,995,086

22,282

Final (including singletons)1

1,129

884.3

23,869,762

26,994


1 Singletons over 1000 bp with BLASTx hits where e < 1 × 10-15 are included in the final consensus

For the first stage all statistics are the average across the four independently sequenced genotypes.

Ness et al. BMC Genomics 2011 12:298   doi:10.1186/1471-2164-12-298

Open Data