Pyrosequencing produces reproducible frequency estimates for KIR transcripts. Pyrosequencing results from four animals are shown. Per animal, two independent cell preparations were performed, and two independent PCRs were performed per cell preparation. For each replicate, the expression of each KIR is graphed as a percentage of total reads. Bars represent the average and standard deviation among replicates. For animal 225-97 (asterisk), only one cell pellet was available, so only two data points are shown.
Moreland et al. BMC Genomics 2011 12:295 doi:10.1186/1471-2164-12-295