Experimental Design. A figure showing the design of the three experiments evaluated here. Biological replicates are separate individuals used for library construction. Technical replicates for the D. melanogaster female heads and D. simulans male heads data are a single library run on multiple lanes. For D. melanogaster c167 cell lines the exact nature of the technical replication is uncertain.
McIntyre et al. BMC Genomics 2011 12:293 doi:10.1186/1471-2164-12-293