Additional file 1.

Normalization and 454 sequencing of transcriptome libraries. (A) Normalization of library of 0-0.5 hrs old embryos. (B) Normalization of library of 3-6 hrs old embryos. Normalization was assessed by quantitative PCR. Shown are transcript levels of E. balteatus alpha tubulin (Eba-tub) relative to E. balteatus orthologues of hunchback (Eba-hb) and caudal (Eba-cad). Bar heights indicate transcript levels, colors indicate levels in the non-normalized cDNA library (blue), and after normalization with 1 unit DSN (red), 2 units DSN (orange), and 4 units DSN (yellow). (C) Distribution of read lengths of library from 0-0.5 hrs old embryos. (D) Distribution of read lengths of library from 3-6 hrs old embryos. Read length (x-axis) is shown as a function of number of reads (y-axis) before (grey graph) and after removal of pea aphid sequence contaminations (black graph).

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Lemke et al. BMC Genomics 2011 12:278   doi:10.1186/1471-2164-12-278