Research article
Fosmid library end sequencing reveals a rarely known genome structure of marine shrimp Penaeus monodon
1 Institute of Zoology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan, ROC
2 Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, ROC
3 Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350, Taiwan, ROC
4 Center for Medical Genetics, and Genetics Laboratory, Department of Medical Research, Changhua Christian Hospital, Changhua 500, Taiwan, ROC
5 Department of Obstertrics and Gynecology, College of Medicine, National Taiwan University, Taipei 106, Taiwan
6 Department of Energy Joint Genome Institute, Walnut Creek, California 94598, USA
7 Department of Evolution and Ecology, University of California Davis Genome Center, University of California, Davis, California 95616, USA
8 Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7 Minato-ku, Tokyo 108-8477, Japan
BMC Genomics 2011, 12:242 doi:10.1186/1471-2164-12-242
Published: 17 May 2011Additional files
Additional file 1:
Estimates of the P. monodon genome size, as percentage of human DNA.
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Additional file 2:
Insert sizes of representative clones from the P. monodon fosmid library. The first and last lanes of each gel are 36 kb-size markers. The average insert size is 40.8 kb.
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Additional file 3:
Frequency and length distribution of the 20,926 Penaeus monodon fosmid end sequences.
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Additional file 4:
Examples of P. monodon ESTs found to contain a very long stretch of microsatellites (a). Some sets of ESTs derived from the same gene showed copy number variation in the microsatellites they contain (b). Dinucleotide repeats [(TC)50, (TA) 50, (TG)50, and (CG)50] were used as query sequences to search against the Penaeus Genome Database. Only top 10 hits were listed.
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Additional file 5:
Thirty-six PREs were found transcriptionally active via BlastN search against the P. monodon EST dataset (PmTwN) in the Penaeus Genome Database. Only top 3 hits are listed.
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