Phenotypic analysis of Hdac1-deficient embryos. (A) Scatter plot of probe intensities from hdac1 mutant versus sibling comparisons. Up-regulated and down-regulated probes in hdac1 mutants are coloured red or green, respectively. The averaged ratio values are plotted on the y-axis and the averaged expression values are plotted on the x-axis, both of which are expressed in a log10 scale. The threshold for statistically significant differential expression was set at p < 10-5. (B) Analysis of the fold-change distribution of Hdac1-regulated probes identified in (A). (C) Microinjection of Hdac1 morpholinos reduces Hdac1 protein abundance in zebrafish embryos. Western blotting for Hdac1 protein in hdac1 mutant (Mut), wild-type (Sib), Hdac1ATG1 morphant (ATG), Hdac1SPL1 morphant (SPL), Hdac1Control morphant (HCo) and Standard Control morphant (StCo) embryos. (D) Reduced immunostaining for Hdac1 in hdac1 mutant and Hdac1ATG1 morphant embryos. Transverse confocal sections through the hindbrain of 12 hpf, 18 hpf and 27 hpf wild-type, hdac1 mutant and Hdac1ATG1 morphant embryos immunostained for Hdac1 protein (green in upper and lower panels) and counterstained with DAPI (blue, in lower panel only).
Harrison et al. BMC Genomics 2011 12:24 doi:10.1186/1471-2164-12-24