Identification of differentially expressed transcripts. Total RNA was extracted from day 1--2 brains of diapause- and nondiapause-destined pupae. Expression of 12 transcripts selected from the F (A) and R libraries (B) was analyzed by RT-PCR, with actin as a control. Four transcripts from the F library (C) were measured by Northern blot analysis. N, nondiapause-destined individuals; D, diapause-destined individuals. The results are representative of two independent experiments.
Bao and Xu BMC Genomics 2011 12:224 doi:10.1186/1471-2164-12-224