Gene distribution from two SSH libraries. In the F library, diapause-type pupae were used as the "tester", and nondiapause pupae as the "driver" to detect highly expressed genes in diapause-type pupae. By contrast, the "tester" and "driver" were reversed in the R library to detect highly expressed genes in nondiapause-type pupae. The Arabic numerals represent the number of unique sequence at each step of the annotation process. NoBlast, no blast results; NoAnnot, no annotation; Annot, annotation. Data analysis and visualization of results were performed by the Blast2GO software.
Bao and Xu BMC Genomics 2011 12:224 doi:10.1186/1471-2164-12-224