Open Access Highly Accessed Research article

Building a model: developing genomic resources for common milkweed (Asclepias syriaca) with low coverage genome sequencing

Shannon CK Straub1*, Mark Fishbein2, Tatyana Livshultz3, Zachary Foster1, Matthew Parks1, Kevin Weitemier1, Richard C Cronn4 and Aaron Liston1

Author Affiliations

1 Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, Corvallis, Oregon 97331, USA

2 Department of Botany, Oklahoma State University, 104 Life Sciences East, Stillwater, Oklahoma 74078, USA

3 The Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, Pennsylvania 19103, USA

4 Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331, USA

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BMC Genomics 2011, 12:211  doi:10.1186/1471-2164-12-211

Published: 4 May 2011

Additional files

Additional file 1:

Polymorphism detected among Illumina reads for the PhiX sequencing control. A graph showing the proportion of bases differing from the majority base in the Illumina PhiX sequencing control.

Format: PDF Size: 774KB Download file

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Open Data

Additional file 2:

Primer sets designed using BatchPrimer3 for 184 nuclear microsatellite loci in Asclepias syriaca not tested for amplification success. A table of nuclear microsatellite locus primer sets, including predicted product size and repeat motif information.

Format: PDF Size: 143KB Download file

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Open Data

Additional file 3:

List of GI numbers for 19,899 Catharanthus roseus ESTs downloaded from GenBank and assembled into unigenes. List of GenBank numbers for Catharanthus roseus ESTs used in this study.

Format: TXT Size: 194KB Download file

Open Data

Additional file 4:

Asclepias syriaca mitochondrial genome contigs. FASTA file containing sequences of the assembled Asclepias syriaca mitochondrial genome contigs.

Format: FSA Size: 134KB Download file

Open Data

Additional file 5:

Asclepias syriaca nuclear genome de novo assembly contigs. FASTA file containing sequences of the nuclear genome contigs from the Velvet de novo Asclepias syriaca genome assembly.

Format: FSA Size: 1.6MB Download file

Open Data