Photomicrographs showing the motif-dependent chromosomal distribution of the pentanucleotide probes in the salivary gland (a, c, f, g, i, m and n) and neuroblast cells (a, b, d, e, h, j, k, l, m and o) of male and female D. melanogaster after ND-FISH and DAPI staining. Each panel shows individual and/or merged images to facilitate the visualization of the signals (green or red for digoxigenin- and biotin-labelled probes respectively) with respect to the DAPI (blue) banding pattern. In some samples interphase and mitotic neuroblast nuclei are shown in the same panels; both polytene and diploid nuclei are shown in a and m. Mitotic chromosomes are identified as well as polytene regions of interest. An example of two-colour ND-FISH is shown in e. Note that only the diffuse chromocentre (CR) observed in polytene nuclei is enriched in pentanucleotide SSRs, which localize to specific heterochromatic regions in mitotic chromosomes as shown in Figure 4. The arrows point to low intensity signals. Scale bar: 5 μm, except in a and m, in which it represents 25 μm.
Cuadrado and Jouve BMC Genomics 2011 12:205 doi:10.1186/1471-2164-12-205