De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing
Genomics Laboratory, Department of Genetic Engineering, SRM University, Chennai, Tamil Nadu, 603 203, India
BMC Genomics 2011, 12:191 doi:10.1186/1471-2164-12-191Published: 15 April 2011
Additional file 1:
Total RNA isolation and normalized cDNA library construction. Total RNA was isolated from roots (R), mature leaves (L), flowers (F), developing seeds (DS), and embryos (E) of Jatropha curcas (Figure A). Normalized cDNA library was constructed from pooled total RNA and the cDNA inserts were PCR amplified from 10 randomly selected clones and resolved in 1.0% agarose gel electrophoresis with 1.0 kb DNA size markers (Figure B).
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Additional file 2:
Reference mapping statistics. Reference assembly with the partial genomic sequence of jatropha showed mapping of 95.87% of the reads and consensus accuracy was 99.15%.
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Additional file 3:
Contigs from de novo assembly of 383,918 reads from the current study. The contig sequences obtained from the de novo assembly of 3, 83,918 reads from Jatropha curcas were given as FASTA format in TXT file.
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