Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Research article

De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing

Purushothaman Natarajan and Madasamy Parani*

Author Affiliations

Genomics Laboratory, Department of Genetic Engineering, SRM University, Chennai, Tamil Nadu, 603 203, India

For all author emails, please log on.

BMC Genomics 2011, 12:191  doi:10.1186/1471-2164-12-191

Published: 15 April 2011

Additional files

Additional file 1:

Total RNA isolation and normalized cDNA library construction. Total RNA was isolated from roots (R), mature leaves (L), flowers (F), developing seeds (DS), and embryos (E) of Jatropha curcas (Figure A). Normalized cDNA library was constructed from pooled total RNA and the cDNA inserts were PCR amplified from 10 randomly selected clones and resolved in 1.0% agarose gel electrophoresis with 1.0 kb DNA size markers (Figure B).

Format: JPEG Size: 94KB Download file

Open Data

Additional file 2:

Reference mapping statistics. Reference assembly with the partial genomic sequence of jatropha showed mapping of 95.87% of the reads and consensus accuracy was 99.15%.

Format: DOC Size: 32KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Contigs from de novo assembly of 383,918 reads from the current study. The contig sequences obtained from the de novo assembly of 3, 83,918 reads from Jatropha curcas were given as FASTA format in TXT file.

Format: TXT Size: 16.4MB Download file

Open Data

Additional file 4:

Contigs from de novo assembly of 991,050 reads from Sato et al., 2011 [9]. The contig sequences obtained from the de novo assembly of 991,050 reads from Sato et al., 2011 [9] were given as FASTA format in TXT file.

Format: TXT Size: 3.7MB Download file

Open Data