NFI binding correlates with histone H3 methylations: H3K4me3, H3K36me3, H3K9me3. 2881 NFI occupied genes in mouse embryonic fibroblasts and 3 groups of randomly selected genes were defined as in Figure 2. ChIP-Seq data for 4 different histone H3 methylations were obtained from the same cell type (mouse embryonic fibroblasts) . Average ChIP-Seq tag counts were calculated in windows of 50 bp for a region of 5 kb up- and down-stream of the orientated transcription start sites (TSS). Tag counts were normalized globally, as a fold increase over the genome average tag count in a window of 50 bp for the following modifications: A. H3K4me3, B. H3K36me3, C. H3K27me3, and D. H3K9me3. Obtained data points were connected to form a continuous line. Arrows indicate the orientation of transcription in each panel.
Pjanic et al. BMC Genomics 2011 12:181 doi:10.1186/1471-2164-12-181