Table 5

Restriction enzymes with unique in silico digestion pattern of 16S rDNA of different Clostridium species

Clostridium species

Restriction Enzyme (RE)


AluI

BfaI

DpnII

HaeIII

SmaI

Tru9I

RsaI


C. baratii

-a

Ub

-

-

-

U

-


C. butyricum

-

-

U

-

-

-

-


C. chauvoei

U

U

U

U

-

-

-


C. colicanis

-

U

U

U

U

-

-


C. kluyveri

U

U

U

U

U

-

-


C. novyi

-

-

-

-

-

U

U


C. pasteurianum

U

U

-

-

-

-

-


C. perfringens

-

U

-

-

-

-

-


C. sardiniense

-

-

-

-

-

U


C. subterminale

U

-

U

-

-

-

-


C. tetani

-

-

U

U

-

-

-


C. acetobutylicum (CAcI)c

U

U

-

-

-

-

-


C. botulinum (CBoI)c

-

U

-

-

-

-

-


aNo unique digestion pattern was detected

bIt denotes unique in terms of fragment length (nucleotides), order and number

cIn the cases of C. acetobutylicum, C. beijerinckii, C. botulinum and C. sporogenes, no unique pattern could be detected and have been discussed separately. The different groups are based on their phylogenetic relationship (Figure 1). It is further assumed here that C. beijerinckii is a part of population comprised of C. acetobutylicum and similarly C. sporogenes is a part of C. botulinum.

Kalia et al. BMC Genomics 2011 12:18   doi:10.1186/1471-2164-12-18

Open Data