Relative mRNA abundances of AtARF6/8-like ZmARFs in wild-type and pre-MIR167b overexpression lines. A. Identification of pre-MIR167b overexpressing transgenic lines by DNA-based bar gene amplification. Amplification products were separated by agarose gel electrophoresis. B. Quantitative real-time RT-PCR analysis was performed by using cDNAs from roots of 8-day-old seedling of MIR167b transgenic lines (pUBI::MIR167b-22, -36 and -47) and wild type (Zong3).
Xing et al. BMC Genomics 2011 12:178 doi:10.1186/1471-2164-12-178