Flow chart of Pt EST sequence analysis. Gene prediction in the P. triticina EST sequences was done by the EST version of the GeneMarkS program . The initial 2nd order model of protein coding regions were derived automatically; then from the set of predicted genes we selected a reduced set of those that were likely to be fungal genes (see text) and more specific 4th order model for fungal genes was derived. Frameshift detection in ESTs with one or more genes predicted in the same strand were analyzed by the frameshift prediction program GeneTack  (see text).
Xu et al. BMC Genomics 2011 12:161 doi:10.1186/1471-2164-12-161