Figure 1.

Flow chart of Pt EST sequence analysis. Gene prediction in the P. triticina EST sequences was done by the EST version of the GeneMarkS program [25]. The initial 2^nd order model of protein coding regions were derived automatically; then from the set of predicted genes we selected a reduced set of those that were likely to be fungal genes (see text) and more specific 4^th order model for fungal genes was derived. Frameshift detection in ESTs with one or more genes predicted in the same strand were analyzed by the frameshift prediction program GeneTack [28] (see text).