Proposed approach for the selection of suitable reference genes. Rather than starting from a subset of commonly used reference genes, we propose to start with an objective choice of candidate genes based on public microarray data obtained from similar experimental conditions. The second step remains identical, i.e. the validation of several candidates within the RT-qPCR experiment being carried out, and the selection of the most stable ones using algorithms such as GeNorm, Norm finder, or Bestkeeper.
Hruz et al. BMC Genomics 2011 12:156 doi:10.1186/1471-2164-12-156