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Open Access Highly Accessed Research article

Identification and characterization of maize microRNAs involved in the very early stage of seed germination

Liwen Wang, Huaihua Liu, Detao Li and Huabang Chen*

Author Affiliations

State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, PR China

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BMC Genomics 2011, 12:154  doi:10.1186/1471-2164-12-154

Published: 18 March 2011

Additional files

Additional file 1:

Summary of small RNA sequencing date.

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Additional file 2:

Conserved miRNAs in maize. These miRNAs are conserved in maize and have been reported in miRBase. "+"present in our dataset, "-"absent in our dataset. L length. **: miRNA sequences of maize were identical to those in other species; *: maize miRNA sequences were conserved in other species but have variations in some nucleotide positions.

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Additional file 3:

Ten conserved maize miRNAs identified in this study.

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Additional file 4:

Novel miRNAs identified in this study.

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Additional file 5:

Secondary structures of novel miRNA in maize. Red colored letter: mature miRNA sequence; blue colored letter: miRNA* sequence.

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Additional file 6:

Predicted targets for novel miRNAs. MiRNAs targeting the same gene and site are grouped together. The first listed miRNA has the least mismatches. "Mm" stands for the total amount of mismatches between the first mentioned miRNA and the predicted target. "Alignment" visually represents miRNA/mRNA complementary base-pairs and mismatches for the first listed miRNA, with vertical bars and spaces as Watson-Crick base-pairs and mismatches, respectively (G:U wobbles count as mismatches).

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