Table 1

D. tertiolecta transcriptome sequencing and assembly summary

Sequences

Bases (Mbp)


Sequencing

Raw sequencing reads

1,385,389

567.6

Average read length

410 bp

Pre-assembly

Trimmed

352,422

Trashed

20,036

Reads used in assembly

1,365,353

546.6

Average read length

400 bp

1Assembly

Contigs

Reads assembled as contigs

609,149

258

Number of contigs

34,301

Average length of contigs

377 bp

Range of contigs length

86-4,258 bp

Depth on contigs

31

Isotigs

Number of isotigs

33,307

17.8

Average length of isotigs

535 bp

Range of isotigs length

101-4,941 bp

Depth on isotigs

2.1

Singletons

376,482

70.1

Unique sequence

409,789


1While this manuscript was in the review process, Newbler v2.5 became publicly available. We reassembled the D. tertiolecta transcriptome using Newbler v2.5 and the outcomes of assembly in comparison with those obtain from v2.3 are presented as the Additional file 1. The differences observed between the outputs of two versions were not significant to impact the major or minor conclusions of this study, and are consistent with reports from Ewen-Campen et al. [77].

Rismani-Yazdi et al. BMC Genomics 2011 12:148   doi:10.1186/1471-2164-12-148

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